BrdU (Bu20a) Mouse mAb 5292S from Cell Signaling Technology

Product Specs
ItemBrdU (Bu20a) Mouse mAb
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number5292S
Quantity100 µl
ApplicationsIHC-P, IF-IC, FC-FP
ReactivityAll
HostMouse
ClonalityMonoclonal
Research AreaCell Cycle / Checkpoint Control
TargetBrdU
IsotypeIgG1
CloneBu20a

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) JAM-C Is Important for Lens Epithelial Cell Proliferation and Lens Fiber Maturation in Murine Lens DevelopmentInvest Ophthalmol Vis SciDecember 14, 2023Qihang Sun, Jiani Li, Jingyu Ma, Yuxing Zheng, Rong Ju, Xuri Li, Xiangrong Ren, Lijuan Huang, Rongyuan Chen, Xuhua Tan, et al.(1:50, AF1213; R&D Systems), mouse anti-FOXE3 (1:200, sc-377465; Santa Cruz), mouse anti-BrdU (1:200, 5292S; Cell Signaling Technology), rabbit anti-ZO-1 (1:200, 40-2200; Invitrogen), rabbit anti-PAX6 (1:500,

(2) The Role of ZNF275/AKT Pathway in Carcinogenesis and Cisplatin Chemosensitivity of Cervical Cancer Using Patient-Derived Xenograft ModelsCancers (Basel)November 28, 2023Miaomiao Ye, Tingxian Liu, Liqing Miao, Shuangwei Zou, Huihui Ji, Jian’an Zhang, Xueqiong Zhubovine serum albumin) for 1 h. Subsequently, the primary anti-BrdU mouse monoclonal antibody (1:200, #5292; CST) was employed to label cells at 4 °C overnight, followed by secondary antibody incubation for 1

(3) The cancer testes antigen, HORMAD1, limits genomic instability in cancer cells by protecting stalled replication forksThe Journal of biological chemistryNovember 1, 2023Herrera LR, Johnson RA, McGlynn K, Gibbs ZA, Davis AJ, Whitehurst AW.(1:500), IdU (347,580, BD Biosciences) (IF 1:50, DNA fiber assay 1:500), bromodeoxyuridine (BrdU) (5292S, CST) (1:100), Vinculin (sc-73614, Santa Cruz) (1:3000), GAPDH (G8795, Sigma-Aldrich) (1:10,000), MRE11

(4) The Preventive Effects of Salubrinal against Pyrethroid-Induced Disruption of Adult Hippocampal Neurogenesis in MiceInternational Journal of Molecular SciencesOctober 26, 2023Abigail C. Toltin, Abdelmadjid Belkadi, Laura M. Gamba, Muhammad M. Hossainto block nonspecific bindings and then incubated with primary antibodies against BrdU (1:200; cat #5292, Cell Signaling, Danvers, MA, USA), Ki-67 (1:200; cat #550609, BD Pharmingen, San Diego, CA, USA), DCX

(5) Time-sequential fibroblast-to-myofibroblast transition in elastin-variable 3D hydrogel environments by collagen networksBiomaterials ResearchOctober 17, 2023Nhuan T. Do, Sun Young Lee, Yoon Seo Lee, ChaeHo Shin, Daeho Kim, Tae Geol Lee, Jin Gyeong Son, Se-Hwa Kimwas purchased from Advanced Biomatrix (Carlsbad, CA, USA). BrdU primary antibody (Cell signaling, #5292) was purchased from Cell Signaling Technology (MA, USA), and mouse anti-S100A4 primary antibody was

(6) Mettl1-mediated internal m7G methylation of Sptbn2 mRNA elicits neurogenesis and anti-alzheimer’s diseaseCell & BioscienceOctober 1, 2023Qingfeng Li, Hui Liu, Lishi Li, Haomin Guo, Zhihao Xie, Xuejian Kong, Jiamin Xu, Junlin Zhang, Yunxia Chen, Zhongsheng Zhang, et al.anti-beta III Tubulin (1:200; Abcam; ab78078), anti-NeuN (1:200; Abcam; ab104224), anti-BruU (1:200; CST; 5292 S), anti-Doublecortin (1:400; CST; 4604 S). 5-Bromo-2′-deoxyuridine and 5-ethynyl-2′-deoxyuridine labeling Prior

(7) MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regenerationSignal Transduction and Targeted TherapySeptember 15, 2023Jinpeng Du, Wenwei Liao, Haichuan Wang, Guimin Hou, Min Liao, Lin Xu, Jiwei Huang, Kefei Yuan, Xiangzheng Chen, Yong Zengchromogenic reaction was performed using a DAB solution for IHC assays. Primary antibodies were BrdU (cat. 5292; CST), pH3S10 (cat. 06-570; Merck Millipore), and Ki67 (cat. ab16667; Abcam). For IF, primary antibodies

(8) The minichromosome maintenance complex drives esophageal basal zone hyperplasiaJCI insightSeptember 8, 2023Rochman M, Rochman Y, Caldwell JM, Mack LE, Besse JA, Manes NP, Yoon SH, Shoda T, Nita-Lazar A, Rothenberg ME.B1 (Santa Cruz Biotechnology, catalog sc-6217); mouse anti-BrdU (Cell Signaling Technology, catalog 5292); mouse anti-GAPDH and anti-HSP90 (OriGene, catalog TA802519 and TA500494); mouse APC-conjugated anti-BrdU

(9) High-Throughput Drug Screening Revealed That Ciclopirox Olamine Can Engender Gastric Cancer Stem-like CellsCancers (Basel)September 3, 2023Diana Pádua, Paula Figueira, Mariana Pinto, André Filipe Maia, Joana Peixoto, Raquel T. Lima, António Pombinho, Carlos Filipe Pereira, Raquel Almeida, Patrícia Mesquitaby immunofluorescence, as in [7]. The coverslips were incubated with the primary antibody BrdU (Bu20a; diluted 1:200; #5292; Cell Signaling Technology) overnight at 4 °C. The goat anti-mouse Alexa Fluor 594 (diluted 1:150; A11032,

(10) The LIN28B–let‐7–PBK pathway is essential for group 3 medulloblastoma tumor growth and survivalMolecular OncologySeptember 1, 2023Shubin W. Shahab, Christianna M. Roggeveen, Jiarong Sun, Haritha Kunhiraman, Leon F. McSwain, Kyle Juraschka, Sachin A. Kumar, Olivier Saulnier, Michael D. Taylor, Matthew Schniederjan, et al.permeabilized, and the DNA was hydrolyzed with 2 N HCl and incubated with primary anti‐BrdU antibody (CST #5292) overnight, before incubating with fluorescently tagged secondary antibody (Invitrogen‐A11005). For

(11) Trim33 masks a non-transcriptional function of E2f4 in replication fork progressionNature communicationsAugust 23, 2023Rousseau V, Einig E, Jin C, Horn J, Riebold M, Poth T, Jarboui MA, Flentje M, Popov N.The slides were blocked and the DNA fibers were stained with antibodies against IdU (Cell Signaling 5292 + anti-mouse Alexa555) and CldU (Abcam ab6326 + anti-rat Alexa 488). Image acquisition was performed

(12) Improvement of neurological recovery in the insomnia rats by Warming Yang Strategy through targeting SIRT4 by inhibiting inflammation and apoptosisImmun Inflamm DisAugust 8, 2023Yuanyuan Liu, Kaihua Rao, Zhengfeng Li, Chunhua Huangblocked with 5% bovine serum albumin (BSA) for 20 min, and incubated with Brdu primary antibody (1:200, #5292; Cell Signaling) for 6 h. Then, cells were washed with phosphate‐buffered saline (PBS), and cultured

(13) The RNA-binding protein Adad1 is necessary for germ cell maintenance and meiosis in zebrafishPLoS geneticsAugust 1, 2023Islam KN, Ajao A, Venkataramani K, Rivera J, Pathania S, Henke K, Siegfried KR.Ddx4/Vasa at 1:2000 [83], rabbit Sycp3 at 1:200 (NB300-232SS, Novus Biologicals), mouse BrdU at 1:200 (5292S, Cell Signaling Technology), rabbit Caspase-3 at 1:1000 (C8487, Sigma-Aldrich). The testes sections

(14) The role of DYNLT3 in breast cancer proliferation, migration, and invasion via epithelial-to-mesenchymal transitionCancer medicineJuly 1, 2023Wang H, Chen X, Jin Y, Liu T, Song Y, Zhu X, Zhu X.(PBS). The cells were treated with 3% BSA for 40 min, and the mouse anti‐BrdU antibody (CST, 1:500, 5292S) was added to incubate at 4°C overnight. Then, the slides were reacted with anti‐mouse antibody for

(15) Supplementation of n-3 PUFAs in Adulthood Attenuated Susceptibility to Pentylenetetrazol Induced Epilepsy in Mice Fed with n-3 PUFAs Deficient Diet in Early LifeMarine DrugsJune 9, 2023Ying-Cai Zhao, Cheng-Cheng Wang, Xiao-Yue Li, Dan-Dan Wang, Yu-Ming Wang, Chang-Hu Xue, Min Wen, Tian-Tian Zhangsections were incubated with the following primary antibodies: mouse monoclonal anti-BrdU (1:1000, #5292, Cell Signaling Technology, USA) and rabbit polyclonal anti-DCX (1:2000, ab18723, Abcam, Cambridge,

(16) Intestinal stem cell aging at single‐cell resolution: Transcriptional perturbations alter cell developmental trajectory reversed by gerotherapeuticsAging CellMay 1, 2023Jiahn Choi, Michele Houston, Ruixuan Wang, Kenny Ye, Wenge Li, Xusheng Zhang, Derek M. Huffman, Leonard H. Augenlichtusing a mouse‐on‐mouse (M.O.M) kit (Vector Labs BMK‐2202) with an antibody against BrdU (1:200; cat#5292, Cell Signaling) following the manufacturing protocol. Slides were then incubated with biotinylated

(17) PRAME Promotes Cervical Cancer Proliferation and Migration via Wnt/β-Catenin Pathway RegulationCancersMarch 16, 2023Chen X, Jiang M, Zhou S, Chen H, Song G, Wu Y, Zhu X.culturing for 8 h. The cell climbing slides were incubated with the mouse anti-BrdU antibody (1:200, 5292S, CST) at 4 °C overnight, and then, anti-mouse IgG/HRP staining reagent (ZSGBbio, DS-0003, Beijing, China)

(18) Artemisia annua Extract Improves the Cognitive Deficits and Reverses the Pathological Changes of Alzheimer’s Disease via Regulating YAP SignalingInternational Journal of Molecular SciencesMarch 9, 2023Wenshu Zhou, Bingxi Lei, Chao Yang, Marta Silva, Xingan Xing, Hua Yu, Jiahong Lu, Wenhua Zheng(Tau46),4019,CST Iba1,GTX1004,GeneTex GFAP (GA5),3670,CST SOX2,MAB5603,Millipore BrdU (Bu20a) mouse mAb,5292,CST IL6,32064,SAB IL-1beta (3A6),12242s,CST TNF-α (D2D4) XP® Rabbit mAb,11948,CST Anti-SYP/Synaptophysin

(19) Histone acetyltransferase KAT2A modulates neural stem cell differentiation and proliferation by inducing degradation of the transcription factor PAX6The Journal of biological chemistryMarch 1, 2023Dong Z, He W, Lin G, Chen X, Cao S, Guan T, Sun Y, Zhang Y, Qi M, Guo B, et al.DMSO, 1xPBST) for 30 min and then incubated in 1:100 BrdU (Bu20a) Mouse mAb anti-BrdU antibody (CST #5292) at 4 °C for 5 days and washed five times in PBST, 10 min each. Next, the samples were incubated in

(20) pH-driven continuous stem cell production with enhanced regenerative capacity from polyamide/chitosan surfacesMaterials Today BioFebruary 1, 2023Chia-Hsiang Yen, Nai-Chen Cheng, Hao-Ying Hsieh, Ching-Wen Tsai, An-Li Lee, Chien-Yi Lu, Yin-Tzu Chen, Tai-Horng Youngfor 30 min at room temperature. The cells were then incubated in anti-BrdU primary antibody (Bu20a; #5292; Cell Signaling Technology, Danvers, MA, USA) with dilution of 1:1000 overnight at 4 °C. Thoroughly

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BrdU (Bu20a) Mouse mAb

BrdU (Bu20a) Mouse mAb from Cell Signaling Technology

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Cell Signaling Technology

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BrdU (Bu20a) Mouse mAb from Cell Signaling Technology

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